As you know by now, entertainment mogul Queen Latifah, 41 and her longtime lover, Jeanette Jenkins, have separated.

According to a confidential source, Queen Latifah, real name Dana Owens, and the 40-something Jenkins, have a rocky on again, off again relationship. But my source tells me the relationship is definitely off for good this time.

My source said that Jenkins, an avid fitness buff, was the glue that held the relationship together. But the personal trainer to the stars is a bit anal and something of a perfectionist. For Jenkins failure is never an option, and she could no longer tolerate being with a woman who refused to exercise and eat right.

“Jeanette is very image and health conscious. She was tired of watching Dana eat, drink and smoke herself to death,” said my source. She confirmed that Latifah’s ballooning weight and her smoking habit were factors in the break up.

My source said the two women, who purchased a home together in the Hollywood hills in 2009, haven’t lived under the same roof in months. “They’re still friends and they love each other, but they’re pursuing their separate careers,” said my source. Dana is living in a hotel in New York where she’s looking for a home, and Jeanette splits her time between Atlanta and LA where she’s filming a reality TV series with twins Tia and Tamera Mowry.

My source said Latifah is depressed because she thinks she failed Jeanette. She said when Latifah is not in meetings for her new clothing line on HSN, she eats alone in her hotel suite waiting for Jeanette to call. In an effort to maintain contact, Latifah cast her ex in her production company’s TV shows, including VH1’s new urban sex dramady “Single Ladies”.

But Jeanette has told Latifah that their long term relationship is over and there is no chance for a reconciliation.