The video was captured by a surveillance camera positioned about a block away from where 3-year-old Kamille and another child played outside a public housing project on Oct. 12.
The video shows one man walk by the children without stopping. A second man, who has a distinctive limp, walks by and the children are seen leaving with him.
The video has no sound and police say the timestamp is incorrect. Kamille was abducted between 8 p.m. and 8:30 p.m. on Oct. 12. A witness saw a man pull Kamille into a blue Toyota Sequoia SUV that sped away.
Police later located the vehicle that was owned by one of two persons of interest who were detained in the case.
Police say the man with the limp is a suspect in the case. They declined to say if the man is in custody.
"Through our investigation and through statements, we've pretty much been able to identify the children and the second gentleman," Police Chief Patrick Smith said on Friday. "What we're trying to do is close the loop on the third individual in the video who was the first one who walks by as the children are playing."
He added: "We want to see if he has additional information or if he saw something that night that may be critical in this investigation."
A person of interest in the case, Patrick Devone Stallworth, 39, of Birmingham, was detained briefly and released on $500,000 bond.
Stallwort was charged with 4 counts of possession of child pornography with the intent to distribute after images were found on his cell phone.
His girlfriend, Derick Irisha Brown, 29, also of Birmingham, is being held with no bond on unrelated charges of kidnapping her three children.
Crime Stoppers is offering a $33,000 reward for information in the case.